qiagen EpiTect Fast DNA Bisulfite Kit快速亞硫酸氫鹽轉化試劑盒
qiagen EpiTect Fast DNA Bisulfite Kit快速亞硫酸氫鹽轉化試劑盒
EpiTect Fast Bisulfite Conversion Kits可在少至30分鐘內將未甲基化的胞嘧啶高效轉化成尿嘧啶，起始材料選擇也有出色的靈活性。簡(jiǎn)化的樣本裂解操作流程和直接的DNA亞硫酸氫鹽轉化使轉化過(guò)程快速、省力，樣本包括全血、培養細胞、組織樣本、基因組DNA和福爾馬林固定石蠟包埋（FFPE）切片。創(chuàng )新的DNA Protect技術(shù)確保有效的DNA變性而不會(huì )降解，得到高產(chǎn)量DNA和可重復性結果。此外，轉化DNA可在少至10 µl洗脫緩沖液中洗脫，得到高濃度DNA。
qiagen EpiTect Fast DNA Bisulfite Kit快速亞硫酸氫鹽轉化試劑盒
Performance
高效亞硫酸氫鹽轉化
在甲基化分析中，對DNA亞硫酸氫鹽處理，將未甲基化的胞嘧啶轉化成胞嘧啶是zui關(guān)鍵的步驟，因為反應效率會(huì )對下游分析的可靠性造成很大影響。EpiTect Fast Bisulfite Conversion Kits含有的創(chuàng )新型Bisulfite Solution可進(jìn)行超快速、高效的亞硫酸氫鹽轉化，甚至可處理難分析的樣本，如來(lái)自女性樣本的X染色體基因組DNA，因為2個(gè)X染色體中有一個(gè)是高度甲基化的（參見(jiàn)"Highly efficient cytosine conversion of X chromosome DNA"）。焦磷酸測序對胞嘧啶轉化定量分析表明，與其他供應商的試劑盒相比，EpiTect Fast Bisulfite Conversion Kit的轉化效果明顯更好，特別是當起始材料的量很有*（參見(jiàn)"Superior bisulfite conversion"）。
訂購信息：
Cat No./ID: 59802 EpiTect Fast Bisulfite Kit (10) Log in Trial kit for 10 preps: Deparaffinization Solution, Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59824 EpiTect Fast DNA Bisulfite Kit (50) Log in For 50 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59826 EpiTect Fast DNA Bisulfite Kit (200) Log in For 200 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59864 EpiTect Fast LyseAll Bisulfite Kit (50) Log in For 50 preps: Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59866 EpiTect Fast LyseAll Bisulfite Kit (200) Log in For 200 preps: Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
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