Toyobo KOD-PLUS高效保真PCR酶
Toyobo KOD-PLUS高效保真PCR酶
KOD -Plus- is based on DNA polymerase from the hyperthermophilic Archaeon
Thermococcus kodakaraensis KOD11) 2). KOD -Plus- exhibits excellent high PCR fidelity
and efficiency. The enzyme solution of KOD -Plus- contains two types of anti-KOD
DNA polymerase antibodies that inhibit polymerase and 3’??5’ exonuclease activity, thus
allowing for Hot Start PCR3). KOD -Plus- generates blunt-end PCR products, due to
3’??5’ exonuclease (proof-reading) activity.
zui大特征: 優(yōu)良的保真性
KOD -Plus-系列的酶具有強力3‘→5’核酸外切酶活性(Proof-reading活性),相比KOD DNA
polymerase,保真性更高,約為T(mén)aq DNA polymerase的80倍。zui適用于克隆。
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