qiagen 204143實(shí)時(shí)定量PCR試劑盒
qiagen 204143實(shí)時(shí)定量PCR試劑盒
QuantiTect SYBR Green PCR Kit使用SYBR Green I通過(guò)兩步法real-time RT-PCR對cDNA目標序列進(jìn)行高特異性的定量檢測。熱啟動(dòng)和*的實(shí)時(shí)定量PCR緩沖液確保了對cDNA目標序列高特異性和靈敏度的real-time定量檢測。dNTP混合液含有dUTP,可選擇性進(jìn)行UNG處理。為便于使用,預混液可儲存在2–8°C。
qiagen 204143實(shí)時(shí)定量PCR試劑盒
特異性定量檢測。
在Rotor-Gene 3000上使用針對人BAX的QuantiTect Primer Assay和的試劑盒分析稀釋的人白細胞cDNA。熔解曲線(xiàn)分析中出現單峰,NTC反應呈平直曲線(xiàn),說(shuō)明使用QuantiTect Kit和Assay可實(shí)現高特異性定量。NTC:無(wú)模板對照。Norm. Fluoro.:標準化的熒光。
at No./ID: 204141 QuantiTect SYBR Green PCR Kit (40) Trial kit for 40 x 50 µl reactions: 1 ml 2x QuantiTect SYBR Green PCR Master Mix, 2 ml RNase-Free Water |
Cat No./ID: 204143 QuantiTect SYBR Green PCR Kit (200) For 200x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water |
Cat No./ID: 204145 QuantiTect SYBR Green PCR Kit (1000) For 1000 x 50 µl reactions: 25 ml 2x QuantiTect SYBR Green PCR Master Mix, 20 ml RNase-Free Water |
Cat No./ID: 204163 QuantiTect SYBR Green PCR +UNG Kit (200) For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green PCR Master Mix, 100 µl UNG Solution, 2 x 2 ml |